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Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking
H Ismail , D Khalid , D Waseem , MU Ijaz , E Dilshad , IU Haq , MZ Bhatti , S Anwaar , M Ahmed , S Saleem
PLoS One,
2023 - VOLUME 18, ISSUE 11
, p e0286349
DOI: 10.1371/journal.pone.0286349
PSIID: 188650195
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Abstract
OBJECTIVE: Berberis lycium is an indigenous plant of Pakistan that is known for its medicinal properties. In the current study, we investigated the anti-Alzheimer's effect of berberine isolated from Berberis lycium.
METHODS: Root extract of B. lycium was subjected to acetylcholinesterase inhibition assay and column chromatography for bioassays guided isolation of a compound. The neuroprotective and memory improving effects of isolated compound were evaluated by aluminium chloride induced Alzheimer's disease rat model, elevated plus maze (EPM) and Morris water maze (MWM) tests., Levels of dopamine and serotonin in rats brains were determined using HPLC. Moreover, western blot and docking were performed to determine interaction between berberine and β-secretase.
RESULTS: During fractionation, ethyl acetate and methanol (3:7) fraction was collected from solvent mixture of ethyl acetate and methanol. This fraction showed the highest anti-acetylcholinesterase activity and was alkaloid positive. The results of TLC and HPLC analysis indicated the presence of the isolated compound as berberine. Additionally, the confirmation of isolated compound as berberine was carried out using FTIR and NMR analysis. In vivo EPM and MWM tests showed improved memory patterns after berberine treatment in Alzheimer's disease model. The levels of dopamine, serotonin and activity of antioxidant enzymes were significantly (p<0.05) enhanced in brain tissue homogenates of berberine treated group. This was supported by decreased expression of β-secretase in berberine treated rat brain homogenates and good binding affinity of berberine with β-secretase in docking studies. Binding energies for interaction of β-secretase with berberine and drug Rivastigmine is -7.0 kcal/mol and -5.8 kcal/mol respectively representing the strong interactions. The results of docked complex of secretase with berberine and Rivastigmine was carried out using Gromacs which showed significant stability of complex in terms of RMSD and radius of gyration. Overall, the study presents berberine as a potential drug against Alzheimer's disease by providing evidence of its effects in improving memory, neurotransmitter levels and reducing β-secretase expression in the Alzheimer's disease model.
Copyright: © 2023 Ismail et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
References
D Lengel , ZL Romm , A Bostwick , ... , R Raghupathi
Journal of Neurotrauma , 2022 - VOLUME 39, ISSUES 13-14 , pp 979-998.
3
68
Y Kong , L Li , LG Zhao , ... , DD Li
Expert Opinion on Therapeutic Patents , 2022 - VOLUME 32, ISSUE 2 , pp 211-223.
Antidiabetic and antioxidant potential of Alnus nitida leaves in alloxan induced diabetic rats.
M Sajid , MR Khan , H Ismail , ... , SA Shah
Journal of Ethnopharmacology2020 - VOLUME 251 p 112544.
12 0
T Belwal , A Bisht , HP Devkota , ... , J Echeverría
Frontiers in Pharmacology , 2020 - VOLUME 11 , p 41.
Review
32
161
D Waseem , GM Khan , IU Haq , ... , DN Syed
Toxicology and Applied Pharmacology , 2020 - VOLUME 401 , p 115091.
J Mehla , SG Lacoursiere , V Lapointe , ... , MH Mohajerani
Neurobiology of Aging , 2019 - VOLUME 75 , pp 25-37.
Pharmacological effects of berberine on mood disorders.
J Fan , K Zhang , Y Jin , ... , R Cui
Journal of Cellular and Molecular Medicine , 2019 - VOLUME 23, ISSUE 1 , pp 21-28.
Review
27
96
H Ismail , AL Gillespie , D Calderwood , ... , BD Green
Metabolites , 2019 - VOLUME 9, ISSUE 5 , p E97.
10
34
M N , AIBL Research Group , F C , ... , A Pa
Journal of Alzheimer’s Disease and Parkinsonism , 2019 - VOLUME 9, ISSUE 2 , p 462.
1
28
2023 - VOLUME 18, ISSUE 11
H Hoshijima , T Mihara , H Seki , ... , T Shiga
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0250909.
0
53
Y Li , D Wilson , R Grundel , ... , JJ Hellmann
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0262382.
0
54
Elevated expression of TUBA1C in breast cancer predicts poor prognosis
Y Zhao , W Wang , J Li , ... , G Shen
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0263710.
0
19
Psychological morbidities among Nepalese migrant workers to Gulf and Malaysia
A Sharma , R Adhikari , E Parajuli , ... , B Adhikari
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0267784.
0
18
N Prasad , I Chien , T Regan , ... , A Thieme
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0272685.
0
33
JR Smeda , HA Smith , MA Mutschler
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0275112.
0
44
BK Reda , G Molla , A Gedefie , ... , A Shibabaw
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0276033.
0
30
E Hallingstad , D Riser-Espinoza , S Brown
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0277150.
0
4
Mechanical analysis of eight-legged temporary support structure under wave forces
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0277353.
0
1
Quantifying gender bias towards politicians in cross-lingual language models
K Stańczak , S Ray Choudhury , T Pimentel , ... , I Augenstein
PLoS One , 2023 - VOLUME 18, ISSUE 11 , p e0277640.
0
1
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Y Zhang , X Xie , B Chen , ... , G Wang
Genes and Diseases , 2024 - VOLUME 11, ISSUE 2 , pp 1022-1034.
0
33
Molecular Dynamics and Other HPC Simulations for Drug Discovery.
Methods in Molecular Biology , 2024 - VOLUME 2716 , pp 265-291.
Recent Deep Learning Applications to Structure-Based Drug Design.
J Verburgt , A Jain , D Kihara
Methods in Molecular Biology , 2024 - VOLUME 2714 , pp 215-234.
Review
0
46
Molecular Dynamics as a Tool for Virtual Ligand Screening.
G Menchon , L Maveyraud , G Czaplicki
Methods in Molecular Biology , 2024 - VOLUME 2714 , pp 33-83.
Antiviral Drug Target Identification and Ligand Discovery.
Methods in Molecular Biology , 2024 - VOLUME 2714 , pp 85-99.
Protein-Ligand Blind Docking Using CB-Dock2.
Methods in Molecular Biology , 2024 - VOLUME 2714 , pp 113-125.
E Ogunsiji , C Umebese , E Stabentheiner , ... , A Oluwajobi
Plant Signaling and Behavior , 2023 - VOLUME 18, ISSUE 1 , p 2217605.
1
38
A Kausar , N Zahra , H Zahra , ... , PV Prasad
Plant Signaling and Behavior , 2023 - VOLUME 18, ISSUE 1 , p 2186045.
0
33
A Álvarez , D Oliveros , YC Ávila , ... , N Guarnizo
Plant Signaling and Behavior , 2023 - VOLUME 18, ISSUE 1 , p 2178362.
1
39